Journal: Molecules

Article Title: The Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′- dimethylchalcone from Cleistocalyx operculatus Buds on Human Pancreatic Cancer Cell Lines

doi: 10.3390/molecules24142538

Figure Lengend Snippet: Effect of DMC on caspase activation and PARP degradation protein expression in PANC-1 cells. Cells were treated with DMC (3–30 µM) for 48 h. Protein 50 µg/lane from cells lysates were electrophoresed on SDS-PAGE gels, then transferred to total blot PVDF membranes. β-Actin was used as a control, (–), 0.1% DMSO-treated cells. The experiments were carried out in three replicates.

Article Snippet: Protein contents were normalized by reprobing the same membrane with anti-β-actin detection; previously used membranes were soaked in stripping buffer (Gene Bio-Application Ltd., Yavne, Israel) at room temperature for 20 min.

Techniques: Activation Assay, Expressing, SDS Page

Journal: Molecules

Article Title: The Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′- dimethylchalcone from Cleistocalyx operculatus Buds on Human Pancreatic Cancer Cell Lines

doi: 10.3390/molecules24142538

Figure Lengend Snippet: Effect of DMC on Bcl-2, Bax and Cyt-c protein expression in PANC-1 cells. Cells were treated with DMC (3–30 µM) for 48 h. Protein 50 µg/lane from cells lysates were electrophoresed on SDS-PAGE gels, then transferred to total blot PVDF membranes. β-Actin was used as a control, (–) 0.1% DMSO-treated cells. The experiments were carried out in three replicates. * P < 0.05 and P < 0.01 compared with control group.

Article Snippet: Protein contents were normalized by reprobing the same membrane with anti-β-actin detection; previously used membranes were soaked in stripping buffer (Gene Bio-Application Ltd., Yavne, Israel) at room temperature for 20 min.

Techniques: Expressing, SDS Page

Journal: European Journal of Medical Research

Article Title: Methylated APC and GSTP1 genes in serum DNA correlate with the presence of circulating blood tumor cells and are associated with a more aggressive and advanced breast cancer disease

doi: 10.1186/2047-783X-15-7-277

Figure Lengend Snippet: Frequencies of methylated genes, CTC and combinations in association to clinico-pathological features.

Article Snippet: The genes were SCGB2A2 Hs00267190_m1 (MGB1) and SPDEF Hs00171942_m1 (Assays on Demand, Applied Bio systems); 2 μl of the cDNA from the AdnaGen Detect RT-Reaction were used as template for each gene.

Techniques: Methylation, Control

Journal: European Journal of Medical Research

Article Title: Methylated APC and GSTP1 genes in serum DNA correlate with the presence of circulating blood tumor cells and are associated with a more aggressive and advanced breast cancer disease

doi: 10.1186/2047-783X-15-7-277

Figure Lengend Snippet: Frequencies of methylated genes in association to circulating tumor cells.

Article Snippet: The genes were SCGB2A2 Hs00267190_m1 (MGB1) and SPDEF Hs00171942_m1 (Assays on Demand, Applied Bio systems); 2 μl of the cDNA from the AdnaGen Detect RT-Reaction were used as template for each gene.

Techniques: Methylation